Abstract: In order to study the effect of different levels of noise to miners' physiological indexes and behavior ability, as well as to study the impact of changes of physiological indexes caused by noise on behavior ability, the test system of miners' physiological indicators and behavioral ability was constructed. The noise was divided into four levels:45 dB, 65 dB, 85 dB and 95 dB, and miners were tested in every case, the physiological indicators including respiratory rate, heart rate, skin electrical value, as well as spatial behavioral ability index including response time, the number of errors were collected synchronously. The experimental data was tested by SPSS20.0, and the structural equation modeling was built by AMOS8.0. The results show that the behavioral ability of miners in the strong noise environment of 85 dB and 95 dB is significantly lower than that in the low noise environment; when the noise increases from 45 dB to 65 dB, the increase of noise doesn't have a significant effect on the error response time and respiration rate of the miners, but has a significant effect on the response time, the number of errors, the heart rate and the skin conductivity level; the effect weight of changes of miners' physiological indexes causes by noise on behavior ability is in the order of skin electrical value, respiratory frequency and heart rate.