Abstract: A methane-oxidizing bacteria belong to Type ⅠMethylomarinum was isolated from return air roadway of coal mine soil, based on the experimental equipment of methane adsorption by microorganism degradation granular coal, the variation of methane degradation rate with degradation time in the static environment with high gas pressure and low oxygen content was studied, and the effect of ambient temperature on the degradation efficiency of methane was also studied. The experimental results show that the degradation rate of methane increases with the increase of degradation time, it increases rapidly in the early stage and then slowly, and finally reaches a certain value, it coincides with the equation of y=ax²+bx+c; methane degradation rate shows obvious difference because methane oxidizing bacteria has different metabolic mechanism under different oxygen concentrations; In the range of 15℃ to 40℃, the methane degradation volume increases at first and then decreases rapidly with the increase of temperature, and coincides with the equation of y=ax³+bx²+cx+d.