Abstract: In order to study the effect of bad emotion on unsafe behaviors of drivers of rubber-tyred car,reduce the incidence of accidents caused by unsafe behaviors of the drivers caused by emotional reasons,a related eye movement experiment was designed.The behavioral responses of drivers in different emotional states were test,about front vehicle,the traffic light and the yellow line,the data such as the number of fixation points and duration of fixation points of the driver during the experiment were statistically analyzed.The results of the study show that:under good mood,the drivers of rubber-tyred car concentrate their attention,the average fixations are more,and the average fixations last longer;under bad mood,the fixation point of the drivers is scattered,the average fixation number is less,and the average fixation duration is short.The intervention countermeasure of the influence of bad emotion on the unsafe behavior of the drivers is put forward.