• 中文核心期刊
  • 中国科技核心期刊
  • RCCSE中国核心学术期刊
  • Scopus, DOAJ, CA, AJ, JST收录期刊
高级检索

鄂尔多斯盆地东缘煤层生物产气与菌群代谢特征

Characteristics of biological gas production and microbial metabolism in coal seams on the eastern margin of the Ordos Basin

  • 摘要: 针对鄂尔多斯盆地东缘煤层气井进入生产中后期出现的产量下降的问题,探索采用生物增产技术作为潜在增产措施。通过采集低产井产出液作为菌源,结合煤样开展生物产气模拟实验,系统分析了代谢中间产物、关键酶活性、微生物群落结构及功能代谢途径等参数的变化特征。实验结果表明,煤样在低产井菌源活化条件下具有一定的产甲烷潜力,单位质量煤甲烷产量达1.15 mL/g;微生物降解煤中有机底物,产生有机酸、醇类和H2等中间产物,作为产甲烷菌底物的酸类物质在体系中被及时降解并相互转化;辅酶F420、氢化酶和纤维素酶活性与生物产气量的变化趋势一致,相关菌群代谢活性同时变化。产气系统中假单胞菌属占比达到57%,甲烷八叠球菌属和产甲烷袋菌属占主导地位,其占比合计超过98%,细菌多样性高于古菌,共同作用参与了煤大分子物质的降解与转化。生物产气过程中产甲烷菌参与了甲烷代谢的3种途径,最大程度上提高了产甲烷效率。乙酸营养型的甲烷产量占51.5%,氢营养型和甲基营养型甲烷产量分别占30.4%、18.1%。发酵系统中优势古菌菌群能够参与不同的甲烷合成途径,最终产生了生物甲烷。

     

    Abstract: As coalbed methane wells in the eastern margin of the Ordos Basin have the problem of production decline that occurs in the middle and later stages, this study explores the adoption of biological stimulation technology as a potential stimulation measures. By collecting the produced fluid from low-yield wells as the bacterial source and conducting biological gas production simulation experiments in combination with coal samples,the variation characteristics of parameters such as metabolic intermediate products, key enzyme activities, microbial community structure and functional metabolic pathways were systematically analyzed. The experimental results show that the coal sample has a certain methane production potential under the activation conditions of bacterial sources in low-yield wells,and the methane yield per unit mass of coal reaches 1. 15 mL/g. Microorganisms degraded organicsubstrates in coal to produce intermediate products such as organic acids,alcohols and H2. The acidic substances serving as substrates for methanogens are promptly degraded and interconverted in the system. The activities of coenzyme F420,hydrogenase,and cellulase are consistent with the changing trend of biological gas production,while the metabolic activities of related microorganisms underwent concurrent changes. In the gas production system,pseudomonas accounted for 57%, while methanococcus and methanofollis are dominant,with a combined proportion exceeding 98%. Bacterial diversity is higher than that of archaea,and they jointly participate in the degradation and transformation of macromolecular substances in coal. In the process of biological gas production,methanogenic bacteria are involved in three pathways of methane metabolism,maximizing the efficiency of methane production. The production of acetotrophic methane accounted for 51. 5%,while the production of hydrogentrophic and methyl-trophic methane accounted for 30. 4% and 18. 1% respectively. The dominant archaeal flora in the fermentation system can participate in different methane synthesis pathways and eventually produce biomethane.

     

/

返回文章
返回