Abstract:
High-efficiency functional flora is an important factor for microbially enhanced coalbed methane. In this paper, the functional fungal flora that degrades aromatic compounds and the fungus-methanogenic flora that degrades coal to produce methane are combined to improve methane production. The succession law of flora structure, intermediate product and coal structure was analyzed, and the mechanism of methanogenesis from lignite degraded by compound flora was studied.The results show that the compound flora can anaerobically degrade the lignite, and the methane production of the coal is 172 μmol/g, which is 1.74 times higher than that none-compound flora. The aromatic degrading fungi
Cladosporium become the dominant fungal genus on the 7th day.
Aspergillus and
Penicillium, which have strong environmental adaptability, become the dominant fungi after gas production. Abundant metabolic intermediate fatty acids and aromatic acids are detected in the fermentation broth on the 7th day, and their proportions decreases by more than 50% at the end of gas production. Aromatic carbon in the coal decreases by 12.27% after degradation, which is significant. The research results provide an effective method for improving the coal degradation ability of methanogenic bacteria and enhancing methanogenesis efficiency.