Abstract: In order to improve the working environment of high-temperature and high-humidity mine and reduce the probability of heatstroke among workers,in the operating environment simulation room, the oral temperature, heart rate and shell temperature of the human body were tested under different temperatures (32℃, 35℃, 38℃) and relative humidity (70%, 90%), the variation tendency and significance of the experimental data were analyzed. The results show that the continuous operation time should not exceed 4 hours under the condition of 32℃ and 90% relative humidity; At 35℃,the continuous operation time should not exceed 3 hours when the relative humidity is 70%, and should not exceed 2 hours when the relative humidity is 90%; when the temperature is 38℃ and the relative humidity is 70%, the continuous operation time should not exceed 1 h, when the relative humidity is 90%, the operation should be stopped immediately. Reasonable measures were put forward from the aspect of self-protection against heat damage of workers in high-temperature and high-humidity mines.